Molecular Identification and Expression Analysis of Carbonic Anhydrase VII in Pufferfish (Takifugu rubripes)

Ocean Science Journal - A carbonic anhydrase VII gene, encoding 277 amino acids, was identified in the intestinal tissue of pufferfish (Takifugu rubripes). The translated protein with an 833-bp...     

Holocene and Eemian climatic optima in the Korean Peninsula based on textural and carbon isotopic records from the stalagmite of the Daeya Cave,South Korea

Textural and stable isotopic records from the absolute-dated stalagmite of the Daeya Cave (DY-1) provide new insights into the climatic evolution of the Korean Peninsula during the Holocene and Eemian climatic optima. The stalagmite yielded ages of 8572 ± 227 to 5907 ± 158 and 1,23,456 ± 535 to 1,19,837 ± 1089 years, which coincide with the Holocene and Eemian climatic optima, respectively. The stalagmite’s δ¹³C record closely resembles previously reported Chinese speleothem δ¹⁸O data. Thus it can be suggested that textural and geochemical results of the DY-1 reflect East Asian monsoon intensity, which is forced by summer insolation patterns in the northern hemisphere. Lighter carbon isotopic compositions, well-developed fibrous calcite crystals, and their relatively faster growth rate in the stalagmite sample are interpreted to reflect the warmest and wettest climate conditions of the Holocene and Eemian interglacials. Both climatic optima took place when insolation was decreasing from its maximum level, temperature in Greenland was highest, and sea level approached its maximum level. These climatic optima also coincide with decreasing Antarctic temperatures. Compared the DY-1 data to other proxies, it is suggested that the Holocene and Eemian climatic optima developed through a balance among boreal insolation, monsoon intensity, and sea level (also continental ice volume), which are the main climatic forcing factors in the northern hemisphere. These trends also follow the bi-polar seesaw mechanism as previously described.     

Metagenomic approach revealed effects of forest thinning on bacterial communities in the forest soil of Mt. Janggunbong,South Korea

The soil microbiome that plays important ecological roles in mountains and forests is influenced by anthropogenic and natural causes. Human activity, particularly harvesting or thinning, affects the soil microbiome in forests by altering environmental conditions, such as vegetation, microclimate, and soil physicochemical properties. The purpose of this study was to investigate the effects on forest thinning on the diversity and composition of the soil bacterial community. From next-generation sequencing results of the 16S rRNA gene, we examined differences in soil bacterial diversity and community composition before and after thinning at Mt. Janggunbong, South Korea. We identified 40 phyla, 103 classes, 192 orders, 412 families, 947 genera, and 3,145 species from the soil samples. Acidobacteria and Proteobacteria were the most dominant bacterial phyla in the forest soil of Mt. Janggunbong. Soil bacterial diversity measures (richness, Shannon diversity index, and evenness) at the phylum level increased after thinning, whereas species-level taxonomic richness decreased after thinning. Thinning provided new opportunities for bacterial species in Chloroflexi, Verrucomicrobia, Nitrospirae, and other nondominant bacterial taxa, especially for those not found in Mt. Janggunbong before thinning, to settle and adapt to the changing environment. Our results suggested that thinning affected the diversity and composition of soil bacterial communities in forests and mountains.     

Evidence for significant clockwise rotations of the Korean Peninsula during Cretaceous

In an effort to evaluate the Cretaceous magnetostratigraphy for the Korean Peninsula and to establish the tectonic coherence of its various elements, we collected paleomagnetic data from 121 samples from 20 sites within the Chilgok Formation (108.3–109.9 Ma) in the Gyeongsang Basin. Together with previously published data, we evaluate the results from a total of 163 sites in the basin.We combine our age model with results from recent stratigraphic, paleomagnetic and radiometric geochemical studies. In this study, we found that two distinct declination shifts decrease with younging direction, indicating two clockwise rotational events of the Korean Peninsula with respect to the Eurasia continent. The earlier event took place during 130–100 Ma (Phase I, newly termed “Goguryeo Disturbance”) and a later one during 80–50 Ma (Phase III, belonging to “Bulguksa Orogeny”). The mean rotation rate in the interval from 115.2 to 103.8 Ma (Phase I) is about 0.74°/Ma, while the rate from 90.9 to 79.8 Ma (Phase II) is 0.19°/Ma. Based on paleolatitude change during Phase I, we infer that the Korean Peninsula (eastern part of the Sino-Korea Block) migrated southward about 300 km after the complete amalgamation of the Sino-Korea Block into the Eurasian continent resulting in N–S compression within the Korean peninsula and Manchuria. Large-scale strike-slip faults (e.g., Tan-Lu Fault, Okcheon Boundary Fault) were probably rejuvenated in the Sino-Korea Block during Phase I.     

Environmentally associated spatial changes of a macrozoobenthic community in the Saemangeum tidal flat,Korea

Estuarine tidal flats are both ecologically and economically important, hence developing methods to reliably measure ecosystem health is essential. Because benthic fauna play a central role in the food web of tidal flats, in this study we set out to quantitatively describe the intertidal zonation of macro-invertebrates and their associations with specific environmental parameters along three transects in the Saemangeum tidal flat, Korea. The abundance and biomass of intertidal fauna with respect to five environmental parameters (i.e., shore level, mud content, coarse sand content, water content, and organic content) were measured, to identify environmental factors that influence macrofaunal distribution in intertidal soft bottom habitats. A total of 75 species were identified, with dominant species showing distinct zones of distribution along all transects. The number of species recorded in each transect was found to be dependent on sediment characteristics and salinity. Cluster analysis classified the entire study area into three faunal assemblages (i.e., location groups), which were delineated by characteristic species, including (A) ‘Periserrula–Macrophthalmus’, (B) ‘Umbonium–Meretrix’, and (C) ‘Prionospio–Potamocorbula’. Four environmental variables (i.e., shore level, water content, mud content, and organic content) appeared to determine factors that distinguished the three faunal assemblages, based on the discriminant analysis. The faunal assemblage types of the sampled locations were accurately predicted from environmental variables in two discriminant functions, with a prediction accuracy of 98%. It should be noted that the zonation of benthos in the lower section (C) of Sandong had been affected by the construction of a nearby dike, while this parameter had remained essentially unchanged at the other two location groups (A–B). Overall, the zonation of benthos from the Saemangeum tidal flat was explained adequately by the measured environmental variables, implying that faunal assemblages are closely associated with certain combinations of abiotic factors. The identification of such reliable associations may facilitate the development of statistical models to predict faunal distributions based on environmental variables at both local and regional scales. The entire study area was embanked in 2006 (one year after this study), and an integrated plan was set into force to develop claimed land into industrial, residential and agricultural districts, which also included a partial restoration program of the tidal flats located near to the study area.     

Comparison of radiocarbon and OSL dating methods for a Late Quaternary sediment core from Lake Ulaan,Mongolia

Both radiocarbon and optically stimulated luminescence (OSL) dating methods were applied to test their suitability for establishing a chronology of arid-zone lacustrine sediments using a 5.88-m-long core drilled from Lake Ulaan, southern Mongolia. Although the radiocarbon and OSL ages agree in some samples, the radiocarbon ages are older than the corresponding OSL ages at the 550-cm depth horizon (late Pleistocene) and in the 100–300-cm interval (early to late Holocene). In the early to late Holocene, radiocarbon ages are consistently older than OSL ages by 4,100–5,800 years, and in the late Pleistocene by 2,700–3,000 years. Grain-size analysis of early to late Holocene sediments and one late Pleistocene sediment sample (550-cm depth) indicates that eolian processes were the dominant sediment-transport mechanism. Also, two late Pleistocene sediments samples (from 400- to 500-cm depths) are interpreted to have been deposited by both eolian and glaciofluvial processes. Accordingly, the radiocarbon ages that were older than the corresponding OSL ages during the Holocene seem to have been a consequence of the influx of ¹⁴C-deficient carbon delivered from adjacent soils and Paleozoic carbonate rocks by the westerly winds, a process that is also active today. In addition to the input of old reworked carbon by eolian processes, the late Pleistocene sediments were also influenced by old carbon delivered by deglacial meltwater. The results of this study suggest that when eolian sediment transport is suspected, especially in lakes of arid environments, the OSL dating method is superior to the radiocarbon dating method, as it eliminates a common ‘old-carbon’ error problem.     

Adaptive Surface Interference Suppression for Matched-Mode Source Localization

Localizing a quiet submerged target in the presence of loud interfering surface ships is an important problem for matched-field processing (MFP) in shallow water. Typically, a data-driven interference suppression scheme is employed which requires neither prior information of the interferer's location nor filter design optimization and iterative estimation. However, the target and the interferers are usually in motion resulting in spreading or mixing of signal energies in their subspaces, thus making it difficult to determine the interference subspace dimension. In this paper, we exploit the difference in modal amplitudes for surface and submerged sources by eigenanalysis of the modal cross-spectral density matrix (CSDM). Simulation and experimental data results show that the interference subspace can be estimated adaptively and the beam output for the target is enhanced.      

Designation processes for marine protected areas in the coastal wetlands of South Korea

The history of Korean tidal flat management and the process for designating Coastal Wetland Protected Areas (CWPAs) are described. Korean coastal wetlands have a long history of intensive use through reclamation for agricultural and industrial uses in the 20th century. Recently, the management policy is shifting away from intensive use towards the conservation of wetlands. This shift is caused by increasing public awareness of the value of wetlands and strong institutional support from the government. Since the Wetlands Conservation Act was passed in 1999, a total of twelve CWPAs have been designated through both top-down and bottom-up processes. Three designation paths are classified based on the relevant drivers, namely government-driven designations (seven CWPAs), local community driven designations (three CWPAs), and conflict resolution (trade-offs) driven designation (two CWPAs). The lessons learned from the designation of Korean CWPAs is that diversification of designation process could facilitate voluntary participation of local stakeholders and thereby enhance the chance of successful implementation of wise use strategy of tidal flats.     

Seasonal variability of community structure and breeding activity in marine phytal harpacticoid copepods on Ulva pertusa from Pohang,east coast of Korea

Seasonal changes in community structure and reproductive status of phytal harpacticoid copepods in the shallow sublittoral bottom at two sites (Masan-ri and Guryongpo) in Pohang (Korea) are described monthly over a period of 1 year (October 1996 to September 1997). A total of 36 harpacticoid species was identified and the numerically dominant copepods were made up of the families Porcellidiidae and Tisbidae. Although the number of species did not show a seasonal trend, total harpacticoid density revealed a favorable distribution for the warmer season (spring and summer) at both sites. Multiple linear regression analyses showed that univariate indices such as density, evenness and diversity are closely associated with certain environmental parameters. For example, the dominant species fluctuated seasonally in abundance and their maximum densities were found to be temperature- (+ with Porcellidium ofunatense) and nutrient-dependent (+ with Scutellidium longicauda acheloides, + with Zaus unisetosus, and ? with P. wandoensis). In addition, the dominant species appeared to breed year round and their reproductive indices are significantly correlated with some environmental parameters such as temperature (?), pH (+), and phosphate (+). Three species (S. l. acheloides, P. wandoensis, and P. ofunatense) showed maximum density two or three months after their reproductive activity reached a maximum. Overall, the seasonal changes in a phytal harpacticoid community could be explained by combinations of environmental parameters supporting the complexity and biodiversity for this specific group of species in coastal ecosystems.